ABSTRACT
Larotaxel, a new taxane compound prepared by partial synthesis from 10-deacetyl baccatin Ⅲ, is active against tumors. In this research, a selective LC–MS method was developed and validated for the study of degradation kinetics of larotaxel, which was carried out in aqueous solutions with different pH (1.5, 3.0, 5.0, 6.5, 7.4, 9.0, 10 and 11.0) and temperature (0, 25, 37 and 45 °C). The linear range was 0.5–25μg/mL, the intra-and inter-day precisions were less than 7.0%, and accuracy ranged from 97.4–104.5% for each analyte. The observed rate obtained by measuring the remaining intact larotaxel was shown to follow first-order kinetics. The activation energies for degradation were 126.7 and 87.01 kJ/mol at pH 1.5 and 11, respectively. Although larotaxel was stable in pH 5, 6.5 and 7.4 buffers at 37 °C for 24 h during our study, increasing or decreasing the pH of the solutions would decrease its stabilities. Moreover, three main degradation products in alkaline condition were separated by HPLC and identified by Q–TOF–MS. The three degradation products were confirmed as 10-deacetyl larotaxel, 7, 8-cyclopropyl baccatin Ⅲ and 10-deacetyl-7, 8-cyclopropyl baccatin Ⅲ.
ABSTRACT
Objective To investigate and observe the expression of Peroxiredoxin Ⅵ in iechemia cortex of rats and the intervention effect of Xingnao Kaiqiao Acupuncture(XKA) therapy. Methods Through western blotting,we detected the expression of Peroxiredoxin Ⅵ in xingnao kaiqiao group , comparison group, model group and sham operation group in middle cerebral artery occlusion (MCAO)model of rats. Results At 6h, the expression of Peroxiredoxin Ⅵ in xingnao kaiqiao group , comparison group and sham operation group was increased than that in moddle group,of which Xingnao Kaiqiao group increased obviously(P < 0.05). At 24h, as compared with modlegroup, the expression of Peroxiredoxin Ⅵ in xingnao kaiqiao group and comparison group increased significantly (P < 0.05). Conclusion Xingnao Kaiqiao Acupuncture therapy could raise the expression of Peroxiredoxin Ⅵ in focal cerebral ischemia of rats, and the effect is better that of traditional acupuncture therapy in the earlier period.It is benefit to elevating the ability of antioxygen of focal cerebral ischemia of rats.
ABSTRACT
Objective To investigate and observe the expression of Peroxiredoxin Ⅵ in ischemia cortex of rats and the intervention effect of Xingnao Kaiqiao Acupuncture(XKA) therapy.Methods Through western blotting,we detected the expression of Peroxiredoxin Ⅵ in xingnao kaiqiao group,comparison group,model group and sham operation group in middle cerebral artery occlusion(MCAO)model of rats.Results At 6h,the expression of Peroxiredoxin Ⅵ in xingnao kaiqiao group,comparison group and sham operation group was increased than that in moddle group,of which Xingnao Kaiqiao group increased obviously(P